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Crystallization and preliminary crystallographic analysis of the Tob-hCaf1 complex.

ABSTRACT: The Tob/BTG family is a group of antiproliferative proteins that contain two highly homologous regions named Box A and Box B. These proteins all associate with CCR4-associated factor 1 (Caf1), which belongs to the ribonuclease D family of deadenylases. The antiproliferative region of human Tob (residues 1-138) and intact hCaf1 were co-expressed in Escherichia coli, purified and successfully cocrystallized. The crystal belongs to the tetragonal space group I422, with unit-cell parameters a = b = 150.9, c = 113.9 A, and is estimated to contain one heterodimer per asymmetric unit. The crystal diffracted to around 2.6 A resolution.

SUBMITTER: Nishida K

PROVIDER: S-EPMC2344109 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature

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Crystallization and preliminary crystallographic analysis of the Tob-hCaf1 complex.

Nishida Kinya K Horiuchi Masataka M Noda Nobuo N NN Takahasi Kiyohiro K Iwasaki Norimasa N Minami Akio A Inagaki Fuyuhiko F

Acta crystallographica. Section F, Structural biology and crystallization communications 20071130 Pt 12

The Tob/BTG family is a group of antiproliferative proteins that contain two highly homologous regions named Box A and Box B. These proteins all associate with CCR4-associated factor 1 (Caf1), which belongs to the ribonuclease D family of deadenylases. The antiproliferative region of human Tob (residues 1-138) and intact hCaf1 were co-expressed in Escherichia coli, purified and successfully cocrystallized. The crystal belongs to the tetragonal space group I422, with unit-cell parameters a = b = ...[more]

PMID: 18084094

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