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Complex assembly, crystallization and preliminary X-ray crystallographic analysis of the chicken CD8??-BF2*0401 complex.

ABSTRACT: In the process of antigen presentation, the MHCI-CD8 complex is important for immune signal transduction by the activation of cytotoxic T cells. Here, the expression, purification, crystallization and X-ray analysis of the complex of the chicken MHC class I molecule BF2*0401 and CD8?? (CD8??-BF2*0401) are reported. This complex was verified by SDS-PAGE analysis of a CD8??-BF2*0401 crystal, which showed three bands corresponding to the molecular weights of BF2*0401, ?2-microglobulin and CD8?, respectively. The crystal of CD8??-BF2*0401 diffracted to 2.8?Å resolution and belonged to space group P21, with unit-cell parameters a = 90.6, b = 90.8, c = 94.9?Å, ? = 98°. The Matthews coefficient and solvent content were calculated to be 2.88?Å(3)?Da(-1) and ?57.3%, respectively.

SUBMITTER: Liu Y

PROVIDER: S-EPMC4157433 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature

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Complex assembly, crystallization and preliminary X-ray crystallographic analysis of the chicken CD8αα-BF2*0401 complex.

Liu Yanjie Y Chen Rong R Tariq Mansoor M Xia Chun C

Acta crystallographica. Section F, Structural biology communications 20140827 Pt 9

In the process of antigen presentation, the MHCI-CD8 complex is important for immune signal transduction by the activation of cytotoxic T cells. Here, the expression, purification, crystallization and X-ray analysis of the complex of the chicken MHC class I molecule BF2*0401 and CD8αα (CD8αα-BF2*0401) are reported. This complex was verified by SDS-PAGE analysis of a CD8αα-BF2*0401 crystal, which showed three bands corresponding to the molecular weights of BF2*0401, β2-microglobulin and CD8α, res ...[more]

PMID: 25195906

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