Complex assembly, crystallization and preliminary X-ray crystallographic analysis of the bovine CD8??-BoLA-2*02201 complex.

ABSTRACT: In order to clarify the structural characteristics of the bovine MHC class I molecule (BoLA-I) complexed with CD8?? (CD8??-BoLA-I), bovine CD8??, BoLA-I (BoLA-2*02201) and ?2m were expressed and purified, and were then assembled with a peptide derived from Foot-and-mouth disease virus (FMDV-VP1YY9) and crystallized. The crystal diffracted to 1.7?Å resolution and belonged to space group P21, with unit-cell parameters a=53.9, b=103.8, c=61.8?Å, ?=?=90, ?=96°. The asymmetric unit contained one complex, with a Matthews coefficient of 2.41?Å3?Da(-1) and a solvent content of 48.9%. The rotation-function Z-score and translation-function Z-score for molecular replacement were 3.4 and 8.9, respectively. In addition, SDS-PAGE analysis of CD8??-BoLA-I crystals showed three bands corresponding to the molecular weights of BoLA-I heavy chain, ?2m and CD8?. The structure of the CD8??-BoLA-I complex should be helpful in obtaining insight into the interaction between bovine CD8?? and MHC class I molecules. Structure determination of BoLA-2*02201-FMDV-VP1YY9 will be useful in the design of vaccines for foot-and-mouth disease.

SUBMITTER: Wang Z 

PROVIDER: S-EPMC4051527 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature