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Crystallization and preliminary X-ray crystallographic analysis of rabbit L-gulonate 3-dehydrogenase.

ABSTRACT: Rabbit L-gulonate 3-dehydrogenase was crystallized using the oil-microbatch method at 295 K. X-ray diffraction data were collected to 1.70 A resolution from a crystal at 100 K using synchrotron radiation. The crystal belongs to the C-centred monoclinic space group C2, with unit-cell parameters a = 71.81, b = 69.08, c = 65.64 A, beta = 102.7 degrees. Assuming the presence of a monomeric protomer in the asymmetric unit gives a V(M) value of 2.21 A(3) Da(-1) and a solvent content of 44.4%. A cocrystal with NADH, which was isomorphous to the apo form, was also prepared and diffraction data were collected to 1.85 A resolution using Cu Kalpha radiation at 100 K.

SUBMITTER: Asada Y

PROVIDER: S-EPMC2374150 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature

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Crystallization and preliminary X-ray crystallographic analysis of rabbit L-gulonate 3-dehydrogenase.

Asada Yukuhiko Y Kuroishi Chizu C Ukita Yoko Y Sumii Rie R Endo Satoshi S Matsunaga Toshiyuki T Hara Akira A Kunishima Naoki N

Acta crystallographica. Section F, Structural biology and crystallization communications 20080229 Pt 3

Rabbit L-gulonate 3-dehydrogenase was crystallized using the oil-microbatch method at 295 K. X-ray diffraction data were collected to 1.70 A resolution from a crystal at 100 K using synchrotron radiation. The crystal belongs to the C-centred monoclinic space group C2, with unit-cell parameters a = 71.81, b = 69.08, c = 65.64 A, beta = 102.7 degrees. Assuming the presence of a monomeric protomer in the asymmetric unit gives a V(M) value of 2.21 A(3) Da(-1) and a solvent content of 44.4%. A cocrys ...[more]

PMID: 18323616

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