Ontology highlight
ABSTRACT:
SUBMITTER: Fan-Minogue H
PROVIDER: S-EPMC2475577 | biostudies-literature | 2008 Jun
REPOSITORIES: biostudies-literature
Fan-Minogue Hua H Du Ming M Pisarev Andrey V AV Kallmeyer Adam K AK Salas-Marco Joe J Keeling Kim M KM Thompson Sunnie R SR Pestova Tatyana V TV Bedwell David M DM
Molecular cell 20080601 5
Organisms that use the standard genetic code recognize UAA, UAG, and UGA as stop codons, whereas variant code species frequently alter this pattern of stop codon recognition. We previously demonstrated that a hybrid eRF1 carrying the Euplotes octocarinatus domain 1 fused to Saccharomyces cerevisiae domains 2 and 3 (Eo/Sc eRF1) recognized UAA and UAG, but not UGA, as stop codons. In the current study, we identified mutations in Eo/Sc eRF1 that restore UGA recognition and define distinct roles for ...[more]