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ABSTRACT:
SUBMITTER: Pendini NR
PROVIDER: S-EPMC2496860 | biostudies-literature | 2008 Jun
REPOSITORIES: biostudies-literature
Pendini Nicole R NR Polyak Steve W SW Booker Grant W GW Wallace John C JC Wilce Matthew C J MC
Acta crystallographica. Section F, Structural biology and crystallization communications 20080523 Pt 6
Biotin protein ligase from Staphylococcus aureus catalyses the biotinylation of acetyl-CoA carboxylase and pyruvate carboxylase. Recombinant biotin protein ligase from S. aureus has been cloned, expressed and purified. Crystals were grown using the hanging-drop vapour-diffusion method using PEG 8000 as the precipitant at 295 K. X-ray diffraction data were collected to 2.3 A resolution from crystals using synchrotron X-ray radiation at 100 K. The diffraction was consistent with the tetragonal spa ...[more]