Unknown

Dataset Information

0

Purification, crystallization and preliminary crystallographic analysis of biotin protein ligase from Staphylococcus aureus.


ABSTRACT: Biotin protein ligase from Staphylococcus aureus catalyses the biotinylation of acetyl-CoA carboxylase and pyruvate carboxylase. Recombinant biotin protein ligase from S. aureus has been cloned, expressed and purified. Crystals were grown using the hanging-drop vapour-diffusion method using PEG 8000 as the precipitant at 295 K. X-ray diffraction data were collected to 2.3 A resolution from crystals using synchrotron X-ray radiation at 100 K. The diffraction was consistent with the tetragonal space group P4(2)2(1)2, with unit-cell parameters a = b = 93.665, c = 131.95.

SUBMITTER: Pendini NR 

PROVIDER: S-EPMC2496860 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

Purification, crystallization and preliminary crystallographic analysis of biotin protein ligase from Staphylococcus aureus.

Pendini Nicole R NR   Polyak Steve W SW   Booker Grant W GW   Wallace John C JC   Wilce Matthew C J MC  

Acta crystallographica. Section F, Structural biology and crystallization communications 20080523 Pt 6


Biotin protein ligase from Staphylococcus aureus catalyses the biotinylation of acetyl-CoA carboxylase and pyruvate carboxylase. Recombinant biotin protein ligase from S. aureus has been cloned, expressed and purified. Crystals were grown using the hanging-drop vapour-diffusion method using PEG 8000 as the precipitant at 295 K. X-ray diffraction data were collected to 2.3 A resolution from crystals using synchrotron X-ray radiation at 100 K. The diffraction was consistent with the tetragonal spa  ...[more]

Similar Datasets

| S-EPMC2805527 | biostudies-literature
| S-EPMC3729168 | biostudies-literature
| S-EPMC6130426 | biostudies-literature
| S-EPMC2330208 | biostudies-literature
| S-EPMC3614165 | biostudies-literature
| S-EPMC3366794 | biostudies-literature
| S-EPMC3080155 | biostudies-literature
| S-EPMC2998359 | biostudies-literature
| S-EPMC3855734 | biostudies-literature
| S-EPMC2374178 | biostudies-literature