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Photochemical DNA activation.


ABSTRACT: A new photocaged nucleoside was synthesized and incorporated into DNA with the use of standard synthesis conditions. This approach enabled the disruption of specific H-bonds and allowed for the analysis of their contribution to the activity of a DNAzyme. Brief irradiation with nonphotodamaging UV light led to rapid decaging and almost quantitative restoration of DNAzyme activity. The developed strategy has the potential to find widespread application in the light-induced regulation of oligonucleotide function.

SUBMITTER: Lusic H 

PROVIDER: S-EPMC2532984 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature

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Photochemical DNA activation.

Lusic Hrvoje H   Young Douglas D DD   Lively Mark O MO   Deiters Alexander A  

Organic letters 20070421 10


A new photocaged nucleoside was synthesized and incorporated into DNA with the use of standard synthesis conditions. This approach enabled the disruption of specific H-bonds and allowed for the analysis of their contribution to the activity of a DNAzyme. Brief irradiation with nonphotodamaging UV light led to rapid decaging and almost quantitative restoration of DNAzyme activity. The developed strategy has the potential to find widespread application in the light-induced regulation of oligonucle  ...[more]

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