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Photochemical DNA activation.

ABSTRACT: A new photocaged nucleoside was synthesized and incorporated into DNA with the use of standard synthesis conditions. This approach enabled the disruption of specific H-bonds and allowed for the analysis of their contribution to the activity of a DNAzyme. Brief irradiation with nonphotodamaging UV light led to rapid decaging and almost quantitative restoration of DNAzyme activity. The developed strategy has the potential to find widespread application in the light-induced regulation of oligonucleotide function.

SUBMITTER: Lusic H

PROVIDER: S-EPMC2532984 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature

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Photochemical DNA activation.

Lusic Hrvoje H Young Douglas D DD Lively Mark O MO Deiters Alexander A

Organic letters 20070421 10

A new photocaged nucleoside was synthesized and incorporated into DNA with the use of standard synthesis conditions. This approach enabled the disruption of specific H-bonds and allowed for the analysis of their contribution to the activity of a DNAzyme. Brief irradiation with nonphotodamaging UV light led to rapid decaging and almost quantitative restoration of DNAzyme activity. The developed strategy has the potential to find widespread application in the light-induced regulation of oligonucle ...[more]

PMID: 17447773

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