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The microRNA miR-1 regulates a MEF-2-dependent retrograde signal at neuromuscular junctions.

ABSTRACT: We show that miR-1, a conserved muscle-specific microRNA, regulates aspects of both pre- and postsynaptic function at C. elegans neuromuscular junctions. miR-1 regulates the expression level of two nicotinic acetylcholine receptor (nAChR) subunits (UNC-29 and UNC-63), thereby altering muscle sensitivity to acetylcholine (ACh). miR-1 also regulates the muscle transcription factor MEF-2, which results in altered presynaptic ACh secretion, suggesting that MEF-2 activity in muscles controls a retrograde signal. The effect of the MEF-2-dependent retrograde signal on secretion is mediated by the synaptic vesicle protein RAB-3. Finally, acute activation of levamisole-sensitive nAChRs stimulates MEF-2-dependent transcriptional responses and induces the MEF-2-dependent retrograde signal. We propose that miR-1 refines synaptic function by coupling changes in muscle activity to changes in presynaptic function.

SUBMITTER: Simon DJ

PROVIDER: S-EPMC2553566 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature

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The microRNA miR-1 regulates a MEF-2-dependent retrograde signal at neuromuscular junctions.

Simon David J DJ Madison Jon M JM Conery Annie L AL Thompson-Peer Katherine L KL Soskis Michael M Ruvkun Gary B GB Kaplan Joshua M JM Kim John K JK

Cell 20080501 5

We show that miR-1, a conserved muscle-specific microRNA, regulates aspects of both pre- and postsynaptic function at C. elegans neuromuscular junctions. miR-1 regulates the expression level of two nicotinic acetylcholine receptor (nAChR) subunits (UNC-29 and UNC-63), thereby altering muscle sensitivity to acetylcholine (ACh). miR-1 also regulates the muscle transcription factor MEF-2, which results in altered presynaptic ACh secretion, suggesting that MEF-2 activity in muscles controls a retrog ...[more]

PMID: 18510933

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2019-03-14 | GSE125490 | GEO

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