Ontology highlight
ABSTRACT:
SUBMITTER: Malawista SE
PROVIDER: S-EPMC2584690 | biostudies-literature | 2008 Nov
REPOSITORIES: biostudies-literature
Malawista Stephen E SE de Boisfleury Chevance Anne A van Damme Jo J Serhan Charles N CN
Proceedings of the National Academy of Sciences of the United States of America 20081107 46
We found exaggerated chemotaxis in plasma treated with EDTA and thought that the EDTA might itself be inhibiting a tonic inhibitor(s) of chemotaxis. Our plasma fractionations suggested that evidence should be sought for a lipid moiety carrying this activity, and on spectrometry (LC-MS-MS together with GC-MS analyses), the biologically active but not the inactive fraction contained oleic and arachidonic acids. Because fatty acids are largely protein bound, we flooded plasma preparations with deli ...[more]