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Crystal structures of DNA/RNA repair enzymes AlkB and ABH2 bound to dsDNA.

ABSTRACT: Escherichia coli AlkB and its human homologues ABH2 and ABH3 repair DNA/RNA base lesions by using a direct oxidative dealkylation mechanism. ABH2 has the primary role of guarding mammalian genomes against 1-meA damage by repairing this lesion in double-stranded DNA (dsDNA), whereas AlkB and ABH3 preferentially repair single-stranded DNA (ssDNA) lesions and can repair damaged bases in RNA. Here we show the first crystal structures of AlkB-dsDNA and ABH2-dsDNA complexes, stabilized by a chemical cross-linking strategy. This study reveals that AlkB uses an unprecedented base-flipping mechanism to access the damaged base: it squeezes together the two bases flanking the flipped-out one to maintain the base stack, explaining the preference of AlkB for repairing ssDNA lesions over dsDNA ones. In addition, the first crystal structure of ABH2, presented here, provides a structural basis for designing inhibitors of this human DNA repair protein.

SUBMITTER: Yang CG

PROVIDER: S-EPMC2587245 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature

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Crystal structures of DNA/RNA repair enzymes AlkB and ABH2 bound to dsDNA.

Yang Cai-Guang CG Yi Chengqi C Duguid Erica M EM Sullivan Christopher T CT Jian Xing X Rice Phoebe A PA He Chuan C

Nature 20080401 7190

Escherichia coli AlkB and its human homologues ABH2 and ABH3 repair DNA/RNA base lesions by using a direct oxidative dealkylation mechanism. ABH2 has the primary role of guarding mammalian genomes against 1-meA damage by repairing this lesion in double-stranded DNA (dsDNA), whereas AlkB and ABH3 preferentially repair single-stranded DNA (ssDNA) lesions and can repair damaged bases in RNA. Here we show the first crystal structures of AlkB-dsDNA and ABH2-dsDNA complexes, stabilized by a chemical c ...[more]

PMID: 18432238

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