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RNA bandages for photoregulating in vitro protein synthesis.

ABSTRACT: 'RNA bandages' are composed of two 6-12-mer 2'-OMe RNA strands complementary to a mRNA target that are joined by a photocleavable linker. These tandem oligonucleotides typically exhibit much higher affinity for the mRNA than the individual strands. An RNA bandage with binding arms of different lengths and a 4-base gap blocked translation in vitro of GFP mRNA; subsequent near-UV irradiation restored translation. This provides a general method of photomodulating hybridization for a variety of oligonucleotide-based technologies.

SUBMITTER: Richards JL

PROVIDER: S-EPMC2593108 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature

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RNA bandages for photoregulating in vitro protein synthesis.

Richards Julia L JL Tang XinJing X Turetsky Anna A Dmochowski Ivan J IJ

Bioorganic & medicinal chemistry letters 20080930 23

'RNA bandages' are composed of two 6-12-mer 2'-OMe RNA strands complementary to a mRNA target that are joined by a photocleavable linker. These tandem oligonucleotides typically exhibit much higher affinity for the mRNA than the individual strands. An RNA bandage with binding arms of different lengths and a 4-base gap blocked translation in vitro of GFP mRNA; subsequent near-UV irradiation restored translation. This provides a general method of photomodulating hybridization for a variety of olig ...[more]

PMID: 18926697

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