Ontology highlight
ABSTRACT:
SUBMITTER: Quiroz-Valenzuela S
PROVIDER: S-EPMC2614477 | biostudies-literature | 2008 Dec
REPOSITORIES: biostudies-literature
Quiroz-Valenzuela Soledad S Sukuru Sai Chetan K SC Hausinger Robert P RP Kuhn Leslie A LA Heller William T WT
Archives of biochemistry and biophysics 20080918 1
Conformational changes of Klebsiella aerogenes urease apoprotein (UreABC)(3) induced upon binding of the UreD and UreF accessory proteins were examined by a combination of flexibility analysis, mutagenesis, and small-angle X-ray scattering (SAXS). ProFlex analysis of urease provided evidence that the major domain of UreB can move in a hinge-like motion to account for prior chemical cross-linking results. Rigidification of the UreB hinge region, accomplished through a G11P mutation, reduced the e ...[more]