Unknown

Dataset Information

0

Pref-1 regulates mesenchymal cell commitment and differentiation through Sox9.


ABSTRACT: Pref-1 is an EGF repeat-containing transmembrane protein that produces a biologically active soluble form by TACE-mediated cleavage. Although Pref-1 inhibition of adipogenesis has been well established, the specific target(s) of Pref-1 or the Pref-1 function in mesenchymal cell commitment/differentiation are not known. Here, we show that Sox9 downregulation is required for adipocyte differentiation and that Pref-1 inhibits adipocyte differentiation through upregulating Sox9 expression. Sox9 directly binds to the promoter regions of C/EBPbeta and C/EBPdelta to suppress their promoter activity, preventing adipocyte differentiation. Furthermore, we also show that, by inducing Sox9, Pref-1 promotes chondrogenic induction of mesenchymal cells but prevents chondrocyte maturation as well as osteoblast differentiation, with supporting in vivo evidence in Pref-1 null and Pref-1 transgenic mice. Thus, Sox9 is a Pref-1 target, and Pref-1 directs multipotent mesenchymal cells to the chondrogenic lineage but inhibits differentiation into adipocytes as well as osteoblasts and chondrocytes.

SUBMITTER: Wang Y 

PROVIDER: S-EPMC2673480 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

Pref-1 regulates mesenchymal cell commitment and differentiation through Sox9.

Wang Yuhui Y   Sul Hei Sook HS  

Cell metabolism 20090301 3


Pref-1 is an EGF repeat-containing transmembrane protein that produces a biologically active soluble form by TACE-mediated cleavage. Although Pref-1 inhibition of adipogenesis has been well established, the specific target(s) of Pref-1 or the Pref-1 function in mesenchymal cell commitment/differentiation are not known. Here, we show that Sox9 downregulation is required for adipocyte differentiation and that Pref-1 inhibits adipocyte differentiation through upregulating Sox9 expression. Sox9 dire  ...[more]

Similar Datasets

| S-EPMC3140487 | biostudies-literature
| S-EPMC2581985 | biostudies-literature
| S-EPMC5881491 | biostudies-literature
| S-EPMC4803524 | biostudies-literature
| S-EPMC2064470 | biostudies-literature
| S-EPMC3285336 | biostudies-literature
| S-EPMC4179489 | biostudies-literature
| S-EPMC6630613 | biostudies-literature
2024-09-20 | PXD046457 | Pride
| S-EPMC2442161 | biostudies-literature