Identification of EMS-induced mutations in Drosophila melanogaster by whole-genome sequencing.

ABSTRACT: Next-generation methods for rapid whole-genome sequencing enable the identification of single-base-pair mutations in Drosophila by comparing a chromosome bearing a new mutation to the unmutagenized sequence. To validate this approach, we sought to identify the molecular lesion responsible for a recessive EMS-induced mutation affecting egg shell morphology by using Illumina next-generation sequencing. After obtaining sufficient sequence from larvae that were homozygous for either wild-type or mutant chromosomes, we obtained high-quality reads for base pairs composing approximately 70% of the third chromosome of both DNA samples. We verified 103 single-base-pair changes between the two chromosomes. Nine changes were nonsynonymous mutations and two were nonsense mutations. One nonsense mutati

SUBMITTER: Blumenstiel JP 

PROVIDER: S-EPMC2674820 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature