Effect of freezing on PCR amplification of 16S rRNA genes from microbes associated with black band disease of corals.
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ABSTRACT:
SUBMITTER: Sekar R
PROVIDER: S-EPMC2675240 | biostudies-literature | 2009 Apr
REPOSITORIES: biostudies-literature
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Effect of freezing on PCR amplification of 16S rRNA genes from microbes associated with black band disease of corals.
Sekar Raju R Kaczmarsky Longin T LT Richardson Laurie L LL
Applied and environmental microbiology 20090227 8
Molecular analysis of black band disease of corals revealed that samples frozen immediately after collection yielded more proteobacterial 16S rRNA sequences, while unfrozen samples produced more cyanobacterial and sulfur-oxidizing bacterial sequences. These results suggest the need to use multiple approaches for preparation of samples to characterize this complex polymicrobial disease. ...[more]
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