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Rapid group-, serotype-, and vaccine strain-specific identification of poliovirus isolates by real-time reverse transcription-PCR using degenerate primers and probes containing deoxyinosine residues.

ABSTRACT: We have adapted our previously described poliovirus diagnostic reverse transcription-PCR (RT-PCR) assays to a real-time RT-PCR (rRT-PCR) format. Our highly specific assays and rRT-PCR reagents are designed for use in the WHO Global Polio Laboratory Network for rapid and large-scale identification of poliovirus field isolates.

SUBMITTER: Kilpatrick DR

PROVIDER: S-EPMC2691077 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature

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Rapid group-, serotype-, and vaccine strain-specific identification of poliovirus isolates by real-time reverse transcription-PCR using degenerate primers and probes containing deoxyinosine residues.

Kilpatrick David R DR Yang Chen-Fu CF Ching Karen K Vincent Annelet A Iber Jane J Campagnoli Ray R Mandelbaum Mark M De Lina L Yang Su-Ju SJ Nix Allan A Kew Olen M OM

Journal of clinical microbiology 20090422 6

We have adapted our previously described poliovirus diagnostic reverse transcription-PCR (RT-PCR) assays to a real-time RT-PCR (rRT-PCR) format. Our highly specific assays and rRT-PCR reagents are designed for use in the WHO Global Polio Laboratory Network for rapid and large-scale identification of poliovirus field isolates. ...[more]

PMID: 19386844

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