Ontology highlight
ABSTRACT:
SUBMITTER: Johnson DB
PROVIDER: S-EPMC3201715 | biostudies-literature | 2011 Sep
REPOSITORIES: biostudies-literature
Nature chemical biology 20110918 11
Stop codons have been exploited for genetic incorporation of unnatural amino acids (Uaas) in live cells, but their low incorporation efficiency, which is possibly due to competition from release factors, limits the power and scope of this technology. Here we show that the reportedly essential release factor 1 (RF1) can be knocked out from Escherichia coli by 'fixing' release factor 2 (RF2). The resultant strain JX33 is stable and independent, and it allows UAG to be reassigned from a stop signal ...[more]