Fluorescence/luminescence circadian imaging of complex tissues at single-cell resolution.
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ABSTRACT:
SUBMITTER: Sellix MT
PROVIDER: S-EPMC2896892 | biostudies-literature | 2010 Jun
REPOSITORIES: biostudies-literature
Publications
Fluorescence/luminescence circadian imaging of complex tissues at single-cell resolution.
Sellix Michael T MT Currie Jake J Menaker Michael M Wijnen Herman H
Journal of biological rhythms 20100601 3
The use of luciferase reporter genes together with luminescence detection has enabled high frequency monitoring of molecular circadian clock function in living tissues. With the help of an intensified CCD camera combined with an inverted epifluorescence microscope, the authors have established a new imaging strategy that makes use of transgenic cell type-specific expression of fluorescent proteins to identify cells of interest for subsequent circadian luminescence recording at single-cell resolu ...[more]
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