Ontology highlight
ABSTRACT: BACKGROUND:
In asthma, CD4+ T cells are selectively recruited into the bronchial mucosa. CD4+ T cells consist of different subsets that express lineage-specific transcription factors and play different roles either in initiating and supporting the development of immune response, but also in orchestrating and regulating them. OBJECTIVES:
The aim of our study was to evaluate the effect of T cells-bronchial fibroblasts interaction on CD4+ T cell phenotype. METHODS:
Human bronchial fibroblasts were isolated from mild steroid naïve asthmatics and nonatopic healthy controls. CD4+ T cells were purified from the peripheral blood of healthy and asthmatic subjects. Co-culture of confluent healthy (HF) or asthmatic bronchial fibroblasts (AF) with T cells were performed. CD4+ T cell total RNA was purified and GATA-3, Foxp3 and RORc expression was detected by quantitative PCR. Th17 (IL-17,IL-22) lineage-specific cytokines profile were also evaluated. RESULTS:
Co-culture of T cells with bronchial fibroblasts significantly stimulated RORc in asthmatic T cells only, whereas Foxp3 and GATA-3 were not affected in both asthmatic and healthy T cells. IL-6 and IL-23 expression either by AF and HF were also significantly increased by the coculture when, TGF-? expression was not affected. In CD4+ T cells, IL-17 and IL-22, Th17 lineage-specific cytokines were significantly increased by the coculture with AF. CONCLUSION:
Interaction between bronchial fibroblasts and T cells seems to specifically promote Th17 cells profile in asthma. These results suggest that cellular interactions particularly between T cells and fibroblasts may play a pivotal role in the regulation of the inflammatory response in asthma.
SUBMITTER: Loubaki L
PROVIDER: S-EPMC2900148 | biostudies-literature | 2010 Jan
REPOSITORIES: biostudies-literature