Ontology highlight
ABSTRACT:
SUBMITTER: Cannon J
PROVIDER: S-EPMC2917504 | biostudies-literature | 2010 Aug
REPOSITORIES: biostudies-literature
Cannon Joe J Lohnes Karen K Wynne Colin C Wang Yan Y Edwards Nathan N Fenselau Catherine C
Journal of proteome research 20100801 8
This report demonstrates the application of a capillary LC-LTQ-orbitrap system to provide automated middle-down analysis of proteolytic peptides in the mass range 3000 to 10,000 Da. The novel workflow combines an underutilized method in the orbitrap-high resolution, mass-accurate product ion measurements-with software tailored to search such data (ProSightPC 2.0) and an Asp-selective chemical cleavage approach that generates peptides across an extended mass range. The strategy using high resolut ...[more]