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Elr-type proteins protect Xenopus Dead end mRNA from miR-18-mediated clearance in the soma.


ABSTRACT: Segregation of the future germ line defines a crucial cell fate decision during animal development. In Xenopus, germ cells are specified by inheritance of vegetally localized maternal determinants, including a group of specific mRNAs. Here, we show that the vegetal localization elements (LE) of Xenopus Dead end (XDE) and of several other germ-line-specific, vegetally localized transcripts mediate germ cell-specific stabilization and somatic clearance of microinjected reporter mRNA in Xenopus embryos. The part of XDE-LE critical for somatic RNA clearance exhibits homology to zebrafish nanos1 and appears to be targeted by Xenopus miR-18 for somatic mRNA clearance. Xenopus Elr-type proteins of the vegetal localization complex can alleviate somatic RNA clearance of microinjected XDE-LE and endogenous XDE mRNA. ElrB1 synergizes with Xenopus Dead end protein in the stabilization of XDE-LE mRNA. Taken together, our findings unveil a functional link of vegetal mRNA localization and the protection of germ-line mRNAs from somatic clearance.

SUBMITTER: Koebernick K 

PROVIDER: S-EPMC2941339 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature

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Elr-type proteins protect Xenopus Dead end mRNA from miR-18-mediated clearance in the soma.

Koebernick Katja K   Loeber Jana J   Arthur Patrick Kobina PK   Tarbashevich Katsiaryna K   Pieler Tomas T  

Proceedings of the National Academy of Sciences of the United States of America 20100830 37


Segregation of the future germ line defines a crucial cell fate decision during animal development. In Xenopus, germ cells are specified by inheritance of vegetally localized maternal determinants, including a group of specific mRNAs. Here, we show that the vegetal localization elements (LE) of Xenopus Dead end (XDE) and of several other germ-line-specific, vegetally localized transcripts mediate germ cell-specific stabilization and somatic clearance of microinjected reporter mRNA in Xenopus emb  ...[more]

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