Ontology highlight
ABSTRACT:
SUBMITTER: Iwamoto M
PROVIDER: S-EPMC2943492 | biostudies-literature | 2010 Sep
REPOSITORIES: biostudies-literature
Iwamoto Mari M Björklund Tomas T Lundberg Cecilia C Kirik Deniz D Wandless Thomas J TJ
Chemistry & biology 20100901 9
The ability to make specific perturbations to biological molecules in a cell or organism is a central experimental strategy in modern research biology. We have developed a general technique in which the stability of a specific protein is regulated by a cell-permeable small molecule. Mutants of the Escherichia coli dihydrofolate reductase (ecDHFR) were engineered to be degraded, and, when this destabilizing domain is fused to a protein of interest, its instability is conferred to the fused protei ...[more]