General method for regulating protein stability with light.
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ABSTRACT:
SUBMITTER: Bonger KM
PROVIDER: S-EPMC3906921 | biostudies-literature | 2014 Jan
REPOSITORIES: biostudies-literature
Publications
General method for regulating protein stability with light.
Bonger Kimberly M KM Rakhit Rishi R Payumo Alexander Y AY Chen James K JK Wandless Thomas J TJ
ACS chemical biology 20131108 1
Post-translational regulation of protein abundance in cells is a powerful tool for studying protein function. Here, we describe a novel genetically encoded protein domain that is degraded upon exposure to nontoxic blue light. We demonstrate that fusion proteins containing this domain are rapidly degraded in cultured cells and in zebrafish upon illumination. ...[more]
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