Ontology highlight
ABSTRACT:
SUBMITTER: Vetting MW
PROVIDER: S-EPMC2957485 | biostudies-literature | 2010 Nov
REPOSITORIES: biostudies-literature
Nature chemical biology 20100919 11
The Mycobacterium tuberculosis enzyme Rv2275 catalyzes the formation of cyclo(L-Tyr-L-Tyr) using two molecules of Tyr-tRNA(Tyr) as substrates. The three-dimensional (3D) structure of Rv2275 was determined to 2.0-Å resolution, revealing that Rv2275 is structurally related to the class Ic aminoacyl-tRNA synthetase family of enzymes. Mutagenesis and radioactive labeling suggests a covalent intermediate in which L-tyrosine is transferred from Tyr-tRNA(Tyr) to an active site serine (Ser88) by transes ...[more]