Inactivation of the WASF3 gene in prostate cancer cells leads to suppression of tumorigenicity and metastases.

ABSTRACT:

Background

The WASF3 protein is involved in cell movement and invasion, and to investigate its role in prostate cancer progression we studied the phenotypic effects of knockdown in primary tumors and cell lines.

Methods

ShRNA was used to knockdown WASF3 function in prostate cell lines. Cell motility (scratch wound assay), anchorage independent growth and in vivo tumorigenicity and metastasis were then compared between knockdown and wild-type cells.

Results

Increased levels of expression were seen in high-grade human prostate cancer and in the PC3 and DU145 cell lines. Inactivation of WASF3 using shRNAs reduced cell motility and invasion in these cells and reduced anchorage independent growth in vitro. The loss of motility was accompanied by an associated increase in s

SUBMITTER: Teng Y 

PROVIDER: S-EPMC2965863 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature