Analysis of lipids from crude lung tissue extracts by desorption electrospray ionization mass spectrometry and pattern recognition.

ABSTRACT: A method is described using desorption electrospray ionization (DESI) mass spectrometry (MS) to obtain phospholipid mass spectral profiles from crude lung tissue extracts. The measured DESI mass spectral lipid fingerprints were then analyzed by unsupervised learning principal components analysis (PCA). This combined approach was used to differentiate the effect(s) of two vaccination routes on lipid composition in mouse lungs. Specifically, the two vaccination routes compared were intranasal (i.n.) and intradermal (i.d.) inoculation of the Francisella tularensis live vaccine strain (Ft-LVS). Lung samples of control and LVS-inoculated mice were quickly extracted with a methanol/chloroform solution, and the crude extract was directly analyzed by DESI-MS, with a total turnaround time of less t

SUBMITTER: Basile F 

PROVIDER: S-EPMC2980589 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature