Ontology highlight
ABSTRACT:
SUBMITTER: Lee HK
PROVIDER: S-EPMC2994549 | biostudies-literature | 2010 May
REPOSITORIES: biostudies-literature
Science (New York, N.Y.) 20100501 5979
In neurons, synaptotagmin 1 (Syt1) is thought to mediate the fusion of synaptic vesicles with the plasma membrane when presynaptic Ca2+ levels rise. However, in vitro reconstitution experiments have failed to recapitulate key characteristics of Ca2+-triggered membrane fusion. Using an in vitro single-vesicle fusion assay, we found that membrane-anchored Syt1 enhanced Ca2+ sensitivity and fusion speed. This stimulatory activity of membrane-anchored Syt1 dropped as the Ca2+ level rose beyond physi ...[more]