Ontology highlight
ABSTRACT:
SUBMITTER: Vrljic M
PROVIDER: S-EPMC2928146 | biostudies-literature | 2010 Mar
REPOSITORIES: biostudies-literature
Vrljic Marija M Strop Pavel P Ernst James A JA Sutton R Bryan RB Chu Steven S Brunger Axel T AT
Nature structural & molecular biology 20100221 3
In neurons, SNAREs, synaptotagmin and other factors catalyze Ca(2+)-triggered fusion of vesicles with the plasma membrane. The molecular mechanism of this process, especially the interaction between synaptotagmin and SNAREs, remains an enigma. Here we characterized this interaction by single-molecule fluorescence microscopy and crystallography. The two rigid Ca(2+)-binding domains of synaptotagmin 3 (Syt3) undergo large relative motions in solution. Interaction with SNARE complex amplifies a par ...[more]