Ontology highlight
ABSTRACT:
SUBMITTER: Sigurdsson S
PROVIDER: S-EPMC2994637 | biostudies-literature | 2010 Apr
REPOSITORIES: biostudies-literature
Sigurdsson Stefan S Dirac-Svejstrup A Barbara AB Svejstrup Jesper Q JQ
Molecular cell 20100401 2
During transcript elongation in vitro, backtracking of RNA polymerase II (RNAPII) is a frequent occurrence that can lead to transcriptional arrest. The polymerase active site can cleave the transcript during such backtracking, allowing transcription to resume. Transcript cleavage is either stimulated by elongation factor TFIIS or occurs much more slowly in its absence. However, whether backtracking actually occurs in vivo, and whether transcript cleavage is important to escape it, has been uncle ...[more]