Ontology highlight
ABSTRACT:
SUBMITTER: Micheva KD
PROVIDER: S-EPMC2995697 | biostudies-literature | 2010 Nov
REPOSITORIES: biostudies-literature
Micheva Kristina D KD Busse Brad B Weiler Nicholas C NC O'Rourke Nancy N Smith Stephen J SJ
Neuron 20101101 4
A lack of methods for measuring the protein compositions of individual synapses in situ has so far hindered the exploration and exploitation of synapse molecular diversity. Here, we describe the use of array tomography, a new high-resolution proteomic imaging method, to determine the composition of glutamate and GABA synapses in somatosensory cortex of Line-H-YFP Thy-1 transgenic mice. We find that virtually all synapses are recognized by antibodies to the presynaptic phosphoprotein synapsin I, ...[more]