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Self-assembling RNA nanorings based on RNAI/II inverse kissing complexes.

ABSTRACT: RNA is an attractive biopolymer for nanodesign of self-assembling particles for nanobiotechnology and synthetic biology. Here, we experimentally characterize by biochemical and biophysical methods the formation of thermostable and ribonuclease resistant RNA nanorings previously proposed by computational design. High yields of fully programmable nanorings were produced based on several RNAI/IIi kissing complex variants selected for their ability to promote polygon self-assembly. This self-assembly strategy relying on the particular geometry of bended kissing complexes has potential for developing short interfering RNA delivery agents.

SUBMITTER: Grabow WW

PROVIDER: S-EPMC3036768 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature

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Self-assembling RNA nanorings based on RNAI/II inverse kissing complexes.

Grabow Wade W WW Zakrevsky Paul P Afonin Kirill A KA Chworos Arkadiusz A Shapiro Bruce A BA Jaeger Luc L

Nano letters 20110113 2

RNA is an attractive biopolymer for nanodesign of self-assembling particles for nanobiotechnology and synthetic biology. Here, we experimentally characterize by biochemical and biophysical methods the formation of thermostable and ribonuclease resistant RNA nanorings previously proposed by computational design. High yields of fully programmable nanorings were produced based on several RNAI/IIi kissing complex variants selected for their ability to promote polygon self-assembly. This self-assembl ...[more]

PMID: 21229999

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