Dataset Information

Differential proteomic analysis of platelets suggested possible signal cascades network in platelets treated with salvianolic acid B.

ABSTRACT:

Background

Salvianolic acid B (SB) is an active component isolated from Danshen, a traditional Chinese medicine widely used for the treatment of cardiovascular disorders. Previous study suggested that SB might inhibit adhesion as well as aggregation of platelets by a mechanism involving the integrin ?2?1. But, the signal cascades in platelets after SB binding are still not clear.

Methodology/principal findings

In the present study, a differential proteomic analysis (two-dimensional electrophoresis) was conducted to check the protein expression profiles of rat platelets with or without treatment of SB. Proteins altered in level after SB exposure were identified by MALDI-TOF MS/MS. Treatment of SB caused regulation of 20 proteins such as heat shock-related 70 kDa protein 2 (hsp70), LIM domain protein CLP-36, copine I, peroxiredoxin-2, coronin-1 B and cytoplasmic dynein intermediate chain 2C. The regulation of SB on protein levels was confirmed by Western blotting. The signal cascades network induced by SB after its binding with integrin ?2?1 was predicted. To certify the predicted network, binding affinity of SB to integrin ?2?1 was checked in vitro and ex vivo in platelets. Furthermore, the effects of SB on protein levels of hsp70, coronin-1B and intracellular levels of Ca²+ and reactive oxygen species (ROS) were checked with or without pre-treatment of platelets using antibody against integrin ?2?1. Electron microscopy study confirmed that SB affected cytoskeleton structure of platelets.

Conclusions/significance

Integrin ?2?1 might be one of the direct target proteins of SB in platelets. The signal cascades network of SB after binding with integrin ?2?1 might include regulation of intracellular Ca²+ level, cytoskeleton-related proteins such as coronin-1B and cytoskeleton structure of platelets.

SUBMITTER: Ma C

PROVIDER: S-EPMC3040754 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature

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Publications

Differential proteomic analysis of platelets suggested possible signal cascades network in platelets treated with salvianolic acid B.

Ma Chao C Yao Yan Y Yue Qing-Xi QX Zhou Xin-Wen XW Yang Peng-Yuan PY Wu Wan-Ying WY Guan Shu-Hong SH Jiang Bao-Hong BH Yang Min M Liu Xuan X Guo De-An DA

PloS one 20110217 2

<h4>Background</h4>Salvianolic acid B (SB) is an active component isolated from Danshen, a traditional Chinese medicine widely used for the treatment of cardiovascular disorders. Previous study suggested that SB might inhibit adhesion as well as aggregation of platelets by a mechanism involving the integrin α2β1. But, the signal cascades in platelets after SB binding are still not clear.<h4>Methodology/principal findings</h4>In the present study, a differential proteomic analysis (two-dimensiona ...[more]

PMID: 21379382

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Dataset Information

Differential proteomic analysis of platelets suggested possible signal cascades network in platelets treated with salvianolic acid B.

Background

Methodology/principal findings

Conclusions/significance

Publications

Differential proteomic analysis of platelets suggested possible signal cascades network in platelets treated with salvianolic acid B.

Similar Datasets

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