Ontology highlight
ABSTRACT:
SUBMITTER: Sarrion-Perdigones A
PROVIDER: S-EPMC3131274 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
Sarrion-Perdigones Alejandro A Falconi Erica Elvira EE Zandalinas Sara I SI Juárez Paloma P Fernández-del-Carmen Asun A Granell Antonio A Orzaez Diego D
PloS one 20110707 7
Synthetic Biology requires efficient and versatile DNA assembly systems to facilitate the building of new genetic modules/pathways from basic DNA parts in a standardized way. Here we present GoldenBraid (GB), a standardized assembly system based on type IIS restriction enzymes that allows the indefinite growth of reusable gene modules made of standardized DNA pieces. The GB system consists of a set of four destination plasmids (pDGBs) designed to incorporate multipartite assemblies made of stand ...[more]