Ontology highlight
ABSTRACT:
SUBMITTER: Yuan J
PROVIDER: S-EPMC3064791 | biostudies-literature | 2011 Mar
REPOSITORIES: biostudies-literature
Yuan Jing J Gogakos Tasos T Babina Arianne M AM Söll Dieter D Randau Lennart L
Nucleic acids research 20101117 6
Mature tRNA(His) has at its 5'-terminus an extra guanylate, designated as G(-1). This is the major recognition element for histidyl-tRNA synthetase (HisRS) to permit acylation of tRNA(His) with histidine. However, it was reported that tRNA(His) of a subgroup of α-proteobacteria, including Caulobacter crescentus, lacks the critical G(-1) residue. Here we show that recombinant C. crescentus HisRS allowed complete histidylation of a C. crescentus tRNA(His) transcript (lacking G(-1)). The addition o ...[more]