Ontology highlight
ABSTRACT:
SUBMITTER: Ko JH
PROVIDER: S-EPMC3875453 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Ko Jae-hyeong JH Llopis Paula Montero PM Heinritz Jennifer J Jacobs-Wagner Christine C Söll Dieter D
PloS one 20131230 12
While translational read-through of stop codons by suppressor tRNAs is common in many bacteria, archaea and eukaryotes, this phenomenon has not yet been observed in the α-proteobacterium Caulobacter crescentus. Based on a previous report that C. crescentus and Escherichia coli tRNA(His) have distinctive identity elements, we constructed E. coli tRNA(His) CUA, a UAG suppressor tRNA for C. crescentus. By examining the expression of three UAG codon- containing reporter genes (encoding a β-lactamase ...[more]