Ontology highlight
ABSTRACT:
SUBMITTER: Pan S
PROVIDER: S-EPMC3069143 | biostudies-literature | 2011 Apr
REPOSITORIES: biostudies-literature
Pan Shenmin S Zhao Linlin L Schenkman John B JB Rusling James F JF
Analytical chemistry 20110311 7
Arrays for screening metabolite-generated toxicity utilizing spots containing DNA, enzyme, and electroluminescent (ECL) polymer ([Ru(bpy)(2)PVP(10)](2+)) were extended to include a fully representative set of metabolic enzymes from human and rat liver microsomes, human and rat liver cytosol, and mouse liver S9 fractions. Array use involves two steps: (1) enzyme activation of the test chemical and metabolite reaction with DNA, and then, (2) capture of ECL resulting from DNA damage using a charge ...[more]