Ontology highlight
ABSTRACT:
SUBMITTER: Sauerwald A
PROVIDER: S-EPMC3785136 | biostudies-literature | 2013 Apr
REPOSITORIES: biostudies-literature
Nature structural & molecular biology 20130310 4
Telomerase contains a large RNA subunit, TER, and a protein catalytic subunit, TERT. Whether telomerase functions as a monomer or dimer has been a matter of debate. Here we report biochemical and labeling data that show that in vivo-assembled human telomerase contains two TERT subunits and binds two telomeric DNA substrates. Notably, catalytic activity requires both TERT active sites to be functional, which demonstrates that human telomerase functions as a dimer. We also present the three-dimens ...[more]