Controlled enzymatic synthesis of natural-linkage, defined-length polyubiquitin chains using lysines with removable protecting groups.
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ABSTRACT:
SUBMITTER: Castaneda CA
PROVIDER: S-EPMC3190230 | biostudies-literature | 2011 Feb
REPOSITORIES: biostudies-literature
Publications
Controlled enzymatic synthesis of natural-linkage, defined-length polyubiquitin chains using lysines with removable protecting groups.
Castañeda Carlos A CA Liu Jia J Kashyap Tanuja R TR Singh Rajesh K RK Fushman David D Cropp T Ashton TA
Chemical communications (Cambridge, England) 20110106 7
E2 enzymes catalyze the ATP-dependent polymerization of polyubiquitin chains which function as molecular signals in the regulation of numerous cellular processes. Here we present a method that uses genetically encoded unnatural amino acids to halt and re-start ubiquitin polymerization providing access to natural-linkage, precision-length ubiquitin chains that can be used for biochemical, structural, and dynamics studies. ...[more]
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