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Gas-phase purification enables accurate, multiplexed proteome quantification with isobaric tagging.

ABSTRACT: We describe a mass spectrometry method, QuantMode, which improves accuracy of isobaric tag-based quantification by alleviating the pervasive problem of precursor interference, simultaneous isolation and fragmentation of impurities, through gas-phase purification. QuantMode analysis of a yeast sample 'contaminated' with interfering human peptides showed substantially improved quantitative accuracy compared to a standard scan, with a small loss of spectral identifications. This technique enables large-scale, multiplexed quantitative proteomics using isobaric tagging.

SUBMITTER: Wenger CD

PROVIDER: S-EPMC3205195 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature

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Gas-phase purification enables accurate, multiplexed proteome quantification with isobaric tagging.

Wenger Craig D CD Lee M Violet MV Hebert Alexander S AS McAlister Graeme C GC Phanstiel Douglas H DH Westphall Michael S MS Coon Joshua J JJ

Nature methods 20111002 11

We describe a mass spectrometry method, QuantMode, which improves accuracy of isobaric tag-based quantification by alleviating the pervasive problem of precursor interference, simultaneous isolation and fragmentation of impurities, through gas-phase purification. QuantMode analysis of a yeast sample 'contaminated' with interfering human peptides showed substantially improved quantitative accuracy compared to a standard scan, with a small loss of spectral identifications. This technique enables l ...[more]

PMID: 21963608

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