Ontology highlight
ABSTRACT:
SUBMITTER: Diskin R
PROVIDER: S-EPMC3232316 | biostudies-literature | 2011 Dec
REPOSITORIES: biostudies-literature
Diskin Ron R Scheid Johannes F JF Marcovecchio Paola M PM West Anthony P AP Klein Florian F Gao Han H Gnanapragasam Priyanthi N P PN Abadir Alexander A Seaman Michael S MS Nussenzweig Michel C MC Bjorkman Pamela J PJ
Science (New York, N.Y.) 20111027 6060
Antibodies against the CD4 binding site (CD4bs) on the HIV-1 spike protein gp120 can show exceptional potency and breadth. We determined structures of NIH45-46, a more potent clonal variant of VRC01, alone and bound to gp120. Comparisons with VRC01-gp120 revealed that a four-residue insertion in heavy chain complementarity-determining region 3 (CDRH3) contributed to increased interaction between NIH45-46 and the gp120 inner domain, which correlated with enhanced neutralization. We used structure ...[more]