Ontology highlight
ABSTRACT:
SUBMITTER: Beloglazova N
PROVIDER: S-EPMC3243599 | biostudies-literature | 2011 Oct
REPOSITORIES: biostudies-literature
The EMBO journal 20111018 22
Clustered regularly interspaced short palindromic repeats (CRISPRs) and Cas proteins represent an adaptive microbial immunity system against viruses and plasmids. Cas3 proteins have been proposed to play a key role in the CRISPR mechanism through the direct cleavage of invasive DNA. Here, we show that the Cas3 HD domain protein MJ0384 from Methanocaldococcus jannaschii cleaves endonucleolytically and exonucleolytically (3'-5') single-stranded DNAs and RNAs, as well as 3'-flaps, splayed arms, and ...[more]