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Single-drop optimization of protein crystallization.

ABSTRACT: A completely new crystal-growth device has been developed that permits charting a course across the phase diagram to produce crystalline samples optimized for diffraction experiments. The utility of the device is demonstrated for the production of crystals for the traditional X-ray diffraction data-collection experiment, of microcrystals optimal for data-collection experiments at a modern microbeam insertion-device synchrotron beamline and of nanocrystals required for data collection on an X-ray laser beamline.

SUBMITTER: Meyer A

PROVIDER: S-EPMC3412791 | biostudies-other | 2012 Aug

REPOSITORIES: biostudies-other

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Single-drop optimization of protein crystallization.

Meyer Arne A Dierks Karsten K Hilterhaus Dierk D Klupsch Thomas T Mühlig Peter P Kleesiek Jens J Schöpflin Robert R Einspahr Howard H Hilgenfeld Rolf R Betzel Christian C

Acta crystallographica. Section F, Structural biology and crystallization communications 20120731 Pt 8

A completely new crystal-growth device has been developed that permits charting a course across the phase diagram to produce crystalline samples optimized for diffraction experiments. The utility of the device is demonstrated for the production of crystals for the traditional X-ray diffraction data-collection experiment, of microcrystals optimal for data-collection experiments at a modern microbeam insertion-device synchrotron beamline and of nanocrystals required for data collection on an X-ray ...[more]

PMID: 22869140

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