Ontology highlight
ABSTRACT:
SUBMITTER: Christis C
PROVIDER: S-EPMC3275380 | biostudies-literature | 2012 Feb
REPOSITORIES: biostudies-literature
Christis Chantal C Munro Sean S
The Journal of cell biology 20120130 3
The small G protein Arf1 regulates Golgi traffic and is activated by two related types of guanine nucleotide exchange factor (GEF). GBF1 acts at the cis-Golgi, whereas BIG1 and its close paralog BIG2 act at the trans-Golgi. Peripheral membrane proteins such as these GEFs are often recruited to membranes by small G proteins, but the basis for specific recruitment of Arf GEFs, and hence Arfs, to Golgi membranes is not understood. In this paper, we report a liposome-based affinity purification meth ...[more]