Ontology highlight
ABSTRACT:
SUBMITTER: Marbaix AY
PROVIDER: S-EPMC3308837 | biostudies-literature | 2011 Dec
REPOSITORIES: biostudies-literature
Marbaix Alexandre Y AY Noël Gaëtane G Detroux Aline M AM Vertommen Didier D Van Schaftingen Emile E Linster Carole L CL
The Journal of biological chemistry 20111012 48
The reduced forms of NAD and NADP, two major nucleotides playing a central role in metabolism, are continuously damaged by enzymatic or heat-dependent hydration. We report the molecular identification of the eukaryotic dehydratase that repairs these nucleotides and show that this enzyme (Carkd in mammals, YKL151C in yeast) catalyzes the dehydration of the S form of NADHX and NADPHX, at the expense of ATP, which is converted to ADP. Surprisingly, the Escherichia coli homolog, YjeF, a bidomain pro ...[more]