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Mesofluidic devices for DNA-programmed combinatorial chemistry.

ABSTRACT: Hybrid combinatorial chemistry strategies that use DNA as an information-carrying medium are proving to be powerful tools for molecular discovery. In order to extend these efforts, we present a highly parallel format for DNA-programmed chemical library synthesis. The new format uses a standard microwell plate footprint and is compatible with commercially available automation technology. It can accommodate a wide variety of combinatorial synthetic schemes with up to 384 different building blocks per chemical step. We demonstrate that fluidic routing of DNA populations in the highly parallel format occurs with excellent specificity, and that chemistry on DNA arrayed into 384 well plates proceeds robustly, two requirements for the high-fidelity translation and efficient in vitro evolution of small molecules.

SUBMITTER: Weisinger RM

PROVIDER: S-EPMC3315586 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Mesofluidic devices for DNA-programmed combinatorial chemistry.

Weisinger Rebecca M RM Marinelli Robert J RJ Wrenn S Jarrett SJ Harbury Pehr B PB

PloS one 20120329 3

Hybrid combinatorial chemistry strategies that use DNA as an information-carrying medium are proving to be powerful tools for molecular discovery. In order to extend these efforts, we present a highly parallel format for DNA-programmed chemical library synthesis. The new format uses a standard microwell plate footprint and is compatible with commercially available automation technology. It can accommodate a wide variety of combinatorial synthetic schemes with up to 384 different building blocks ...[more]

PMID: 22479318

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