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Targeted delivery of chemotherapy agents using a liver cancer-specific aptamer.


ABSTRACT: Using antibody/aptamer-drug conjugates can be a promising method for decreasing toxicity, while increasing the efficiency of chemotherapy.In this study, the antitumor agent Doxorubicin (Dox) was incorporated into the modified DNA aptamer TLS11a-GC, which specifically targets LH86, a human hepatocellular carcinoma cell line. Cell viability tests demonstrated that the TLS11a-GC-Dox conjugates exhibited both potency and target specificity. Importantly, intercalating Dox into the modified aptamer inhibited nonspecific uptake of membrane-permeable Dox to the non-target cell line. Since the conjugates are selective for cells that express higher amounts of target proteins, both criteria noted above are met, making TLS11a-GC-Dox conjugates potential candidates for targeted delivery to liver cancer cells.Considering the large number of available aptamers that have specific targets for a wide variety of cancer cells, this novel aptamer-drug intercalation method will have promising implications for chemotherapeutics in general.

SUBMITTER: Meng L 

PROVIDER: S-EPMC3338807 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Targeted delivery of chemotherapy agents using a liver cancer-specific aptamer.

Meng Ling L   Yang Liu L   Zhao Xiangxuan X   Zhang Lucy L   Zhu Haizhen H   Liu Chen C   Tan Weihong W  

PloS one 20120425 4


<h4>Background</h4>Using antibody/aptamer-drug conjugates can be a promising method for decreasing toxicity, while increasing the efficiency of chemotherapy.<h4>Methodology/principal findings</h4>In this study, the antitumor agent Doxorubicin (Dox) was incorporated into the modified DNA aptamer TLS11a-GC, which specifically targets LH86, a human hepatocellular carcinoma cell line. Cell viability tests demonstrated that the TLS11a-GC-Dox conjugates exhibited both potency and target specificity. I  ...[more]

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