WW Domain Containing Transcription Regulator regulates human conjunctiva epithelial cell proliferation via inhibiting TGF? signaling pathway [corrected].

ABSTRACT: PURPOSE: To investigate the role of Tafazzin (TAZ) protein in regulating the proliferation of normal human conjunctiva epithelial cells and epithelial cells from pterygium tissue. METHODS: Conjunctiva epithelial cells were cultured in keratinocytes growth medium and treated with transformation growth factor ? (TGF?) to analyze the expression and translocation of TAZ protein by immunostaining and BrdU analysis. Immortalized conjunctiva epithelial cells (NHC) were treated with TGF?, targeting siRNA, TGF? receptor antibody or TGF? receptor inhibitor, to study the involvement of TAZ and TGF? signaling pathway in conjunctiva cell proliferation by cell adhesion assay. Conjunctiva tissues from a normal human eye and an eye with pterygium disease were collected for histological analyses and western blot to evaluate the TAZ protein expression in vivo. RESULTS: TAZ expression was upregulated in mitotic conjunctiva epithelial cells, proliferating conjunctiva epithelial cells, TGF? treated conjunctiva epithelial cells and human pterygium epithelium. TAZ siRNA induced less conjunctiva epithelial cell growth. Moreover, TGF? receptor antibody and TGF? receptor inhibitor rescued this anti-proliferative effect of TAZ siRNA. CONCLUSIONS: TAZ is involved in human conjunctiva epithelial cells proliferation via regulating TGF? signaling pathway.

SUBMITTER: Tan XW 

PROVIDER: S-EPMC3369895 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature