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Ubiquitylation of the initiator caspase DREDD is required for innate immune signalling.


ABSTRACT: Caspases have been extensively studied as critical initiators and executioners of cell death pathways. However, caspases also take part in non-apoptotic signalling events such as the regulation of innate immunity and activation of nuclear factor-?B (NF-?B). How caspases are activated under these conditions and process a selective set of substrates to allow NF-?B signalling without killing the cell remains largely unknown. Here, we show that stimulation of the Drosophila pattern recognition protein PGRP-LCx induces DIAP2-dependent polyubiquitylation of the initiator caspase DREDD. Signal-dependent ubiquitylation of DREDD is required for full processing of IMD, NF-?B/Relish and expression of antimicrobial peptide genes in response to infection with Gram-negative bacteria. Our results identify a mechanism that positively controls NF-?B signalling via ubiquitin-mediated activation of DREDD. The direct involvement of ubiquitylation in caspase activation represents a novel mechanism for non-apoptotic caspase-mediated signalling.

SUBMITTER: Meinander A 

PROVIDER: S-EPMC3380211 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature

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Ubiquitylation of the initiator caspase DREDD is required for innate immune signalling.

Meinander Annika A   Runchel Christopher C   Tenev Tencho T   Chen Li L   Kim Chan-Hee CH   Ribeiro Paulo S PS   Broemer Meike M   Leulier Francois F   Zvelebil Marketa M   Silverman Neal N   Meier Pascal P  

The EMBO journal 20120501 12


Caspases have been extensively studied as critical initiators and executioners of cell death pathways. However, caspases also take part in non-apoptotic signalling events such as the regulation of innate immunity and activation of nuclear factor-κB (NF-κB). How caspases are activated under these conditions and process a selective set of substrates to allow NF-κB signalling without killing the cell remains largely unknown. Here, we show that stimulation of the Drosophila pattern recognition prote  ...[more]

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