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Tunable reagents for multi-functional bioconjugation: reversible or permanent chemical modification of proteins and peptides by control of maleimide hydrolysis.

ABSTRACT: Controlling maleimide hydrolysis allows the modular construction of bromomaleimide-mediated bioconjugates which are either stable or cleavable in an aqueous, thiol-mediated reducing environment. The application of this methodology to reversible protein biotinylation, the irreversible labeling of peptide disulfide bonds and the assembly of stable, fluorescein-labelled glycoprotein mimics is described.

SUBMITTER: Ryan CP

PROVIDER: S-EPMC3381635 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature

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Tunable reagents for multi-functional bioconjugation: reversible or permanent chemical modification of proteins and peptides by control of maleimide hydrolysis.

Ryan Chris P CP Smith Mark E B ME Schumacher Felix F FF Grohmann Dina D Papaioannou Danai D Waksman Gabriel G Werner Finn F Baker James R JR Caddick Stephen S

Chemical communications (Cambridge, England) 20110405 19

Controlling maleimide hydrolysis allows the modular construction of bromomaleimide-mediated bioconjugates which are either stable or cleavable in an aqueous, thiol-mediated reducing environment. The application of this methodology to reversible protein biotinylation, the irreversible labeling of peptide disulfide bonds and the assembly of stable, fluorescein-labelled glycoprotein mimics is described. ...[more]

PMID: 21465057

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