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A fast response highly selective probe for the detection of glutathione in human blood plasma.

ABSTRACT: A fluorescent probe for glutathione (GSH) detection was developed. Our study indicates a possible mechanism which couples a conjugate addition and micelle-catalyzed large membered ring formation/elimination sequence. This method enables excellent selectivity towards GSH over other biological thiols such as cysteine (Cys) and homocysteine (Hcy). The proposed method is precise with a relative standard deviation (R.S.D) lower than 6% (n = 3) and has been successfully applied to determine GSH in human plasma with recoveries between 99.2% and 102.3%.

SUBMITTER: Guo Y

PROVIDER: S-EPMC3386722 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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A fast response highly selective probe for the detection of glutathione in human blood plasma.

Guo Yixing Y Yang Xiaofeng X Hakuna Lovemore L Barve Aabha A Escobedo Jorge O JO Lowry Mark M Strongin Robert M RM

Sensors (Basel, Switzerland) 20120508 5

A fluorescent probe for glutathione (GSH) detection was developed. Our study indicates a possible mechanism which couples a conjugate addition and micelle-catalyzed large membered ring formation/elimination sequence. This method enables excellent selectivity towards GSH over other biological thiols such as cysteine (Cys) and homocysteine (Hcy). The proposed method is precise with a relative standard deviation (R.S.D) lower than 6% (n = 3) and has been successfully applied to determine GSH in hum ...[more]

PMID: 22778623

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